In the design of food factory, the clean room of food factory not only relies on the method of air filtration, so that the number of biological or non-biological
particles in the air sent into the clean room of food factory is strictly controlled, but also carries out disinfection treatment on the surface of indoor utensils, ground, walls,wallboards and so on. Therefore, biological clean room in addition to meet the requirements of general food factory clean room, its internal materials can also withstand the erosion of various sterilizers.

The air through the sub-high efficiency or high efficiency filter can be regarded as sterile air, but filtration is only a means of sterilization, and there is no sterilization effect, because there are personnel, materials and other sources of pollution in the clean room of the food factory, so as long as there are nutrients needed by microorganisms, it can make microorganisms survive and multiply. Therefore, disinfection and sterilization measures can never be ignored in the design and management of clean room in food factories.

Traditional sterilization methods include ultraviolet sterilization, pharmaceutical sterilization and heating sterilization. These methods are well known and their safety and reliability have been confirmed by long-term practice. But these methods also have their own defects, such as ultraviolet sterilization, the device is more convenient to set up and use, but because of limited penetration capacity, the place where ultraviolet radiation is less than, the sterilization effect is not good, and the lamp life is short, frequent replacement, high operating cost; Chemical reagent sterilization, such as formaldehyde fumigation, troublesome operation, long fumigation time, secondary pollutants, there is a certain harm to the body, fumigation residue attached to the wall and equipment surface of the clean room, need to be cleaned, sometimes improper treatment, in a few days after sterilization, the number of suspended particles will increase; Heating sterilization includes dry heat and wet heat, its disadvantages are high temperature, high energy consumption, some items such as some raw materials, instruments and meters, plastic products, etc., should not be used.

In recent years, ozone sterilization in pharmaceutical products, biological drugs manufacturing process has been increasingly widely used, ozone is a broad-spectrum fungicide, can kill bacteria and spores, viruses, fungi, etc., can destroy endotoxin. Ozone kills bacteria faster than chlorine in water. Ozone sterilization method has been used in some biological clean rooms for disinfection and sterilization of pipes and containers, through the purification of air conditioning system to clean room sterilization, surface disinfection and water disinfection and so on.

Which sterilization method is used in the specific food factory clean room should be determined according to the use of food factory clean room, product production process characteristics and the actual situation of the production equipment used.

Ozone (O3) is the isotrope of oxygen. Ozone is a strong oxidant, and its sterilization process belongs to biochemical oxidation reaction, which decomposes glucose oxidase necessary for glucose oxidation in bacteria. It can act directly with bacteria and viruses to destroy their cells and ribonucleic acid. Ozone is dissolved mold sterilization agent, when the air is contained in a certain concentration of ozone (2 ~ 15) x 10-6], the internal structure of bacteria, viruses and other microorganisms strong oxidative damaging, to kill bacteria breeding, spore, armour hepatitis b virus, such as bacteria, fungi and protozoa cystic can also damage the toxins botox and rickettsia, etc. Ozone has a strong mildew, fishy, smelly, mildew, moth - proof and other functions.

Compared with ultraviolet method and chemical fumigation method, ozone sterilization method has the advantages of convenient operation and use, unlike formaldehyde fumigation method, time-consuming labor, and no secondary pollution cleaning problems; Unlike the dead spots that ultraviolet radiation can cause, the diffusion of ozone can disinfect and sterilize any corner. At present, ozone sterilization method has been used in more than 200 domestic pharmaceutical factories, mainly used for clean room disinfection and sterilization and purification of air conditioning system, pure water and injection water disinfection and sterilization, more than 20 through GMP certification. Ozone sterilization in clean rooms of pharmaceutical factories is generally to install ozone generators in appropriate locations such as purification and air conditioning systems. When disinfecting and sterilizing, close the fresh air inlet and return air discharge air valve, so that the whole disinfected clean room air of the device forms a circulation state through the air duct, and start air sterilization for 1 to 1.5 hours every day.